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KMID : 0362319960210010403
Journal of Korean Academy of Operative Dentistry
1996 Volume.21 No. 1 p.403 ~ p.424
Cytotoxic effect of retrograde filling materials including glass ionmer cement according to cell lines and assay methods


Abstract
Cell culture methods have been used to assess the cytotoxicity of dental materials. Different paramaters are used to monitor cytoxic effects. But it is difficult to compare each investigator's results with different methods. The objective of this
study
was to investigate cytotoxic effect of several retrograde filling materials according to cell lines and assay methods. Cytotoxicity of Bestalloy(Dogmyung. Korea), Prisma APH(Densply International Inc., U.S.A.), Clearfil FII(Kuraray co., Japan),
Fuji
II(GC Co., Japan), Fuji II LC (GC Co., Japan) and IRM (Densply Co., U.S.A.) on L929, 3T3 and KB permanent cell lines was measured. Radiochromium, Lactate dehydrogenase (LDH) release method and colorimetric assays, namely neutral red(NR) and MTT
were
used. Each material was mixed according to the manufacturer's instruction. They were tested as solid and extracted state. Cell culture media were added to each mixed or solid materials then the solution was collected and used as extract
solutions.
Solid Fuji II showed mild cytotoxicity on three cell lines using radiochromium release method. There was no difference in cytotoxicity of extract solution group using radiochromium release method. In colorimetric assay immediate Fuji II group and
all
the IRM groups showed severe cytotoxic effect. Difference in cyctotoxicity was due to rather kinds of cell lines than assay methods. Solid Fuji II and IRM showed mild cytotoxicity on three cell lines. But extract solutions had different cytotoxic
effect
according to cell lines using LDH release assay. Light-cured glass ionomer had mild to moderate degree of cytotoxicity on three cell lines.
Cytotoxicity was affected by specimen prepaton. Sus;ceptibility of each cell ines were also affected by assay emthods. It was suggested that cytotoxicity study using only one cell line and/or assay method might not accurately reflect the real
toxic
nature of dental biomaterials.
KEYWORD
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